The PhD theses of this Research Training Group depend on each other. Each of them requires a certain core expertise but also at least two neighboring expertises for its success.
The Burghardt group brings expertise in the theoretical understanding of light-induced processes in complex molecular systems, including solute-solvent systems, biological systems (e.g., photoswitch-RNA/DNA and chromophore-protein complexes), and materials (e.g., semiconducting polymer materials used in organic photovoltaics). Within the RTG, the group will develop and employ theoretical tools that are specifically adapted to (i) novel spectroscopic wavelength-selective uncaging approaches and (ii) the study of uncaging controlled kinetics.
The PhD students of this project area will acquire in-depth knowledge of the theoretical and computational treatment of wavelength-selective photoexcitation, including the novel double-resonance VIPER approach as well as two-photon absorption techniques. (The latter aspect will involve a close collaboration with the Dreuw group, Heidelberg University.) Furthermore, the students will gain thorough understanding of the combination of electronic structure theory and kinetic approaches to obtain theoretical predictions of efficient uncaging platforms.
Over the last ten years the Heckel group has gained extensive knowledge in the uncaging of DNA and RNA and recently also of small peptides. They have shown how to regulate aptamers, siRNAs and antimiRs with light as well as prepared light-activatable molecular beacons.
The PhD students of this project area will obtain a deep knowledge in the synthesis and characterization of sophisticated photolabile groups and their introduction into biopolymers. They will receive an excellent training in the use of laser, LED and other light sources, synthesis, handling and characterization of DNA, RNA and peptides and in advanced microscopy techniques. They will further understand the principles of computational methods for the prediction of electronic structures as well as the methods used in (ultrafast) optical spectroscopy and super-resolution microscopy.
The Wachtveitl group is specialized in the development and application of time-resolved spectroscopic methods from femtoseconds to seconds optimized for dynamic studies of uncaging. Ultrafast (vibrational) spectroscopy is an especially suitable tool for the time-resolved investigation of reaction pathways and of small fragments generated in the photolysis reaction.
The PhD students of this project area will receive an excellent training in biomolecular spectroscopy both in the development of state of the art methodology and in the applications on biological systems. They will perform femtosecond time-resolved electronic and vibrational spectroscopic experiments and acquire profound knowledge of nonlinear optics and quantum chemistry. They will learn the handling of photolabile compounds, understand the principles of organic synthesis and participate in the design of optimized compounds.
The expertise of the Bredenbeck group lies in femtosecond spectroscopy with a focus on mixed IR-UV/VIS multipulse experiments and two-dimensional IR spectroscopy. The group developed a flexible experimental platform that provides femtosecond pulses between 260 nm and 10.000 nm with arbitrary delays from the femtosecond to the millisecond range, which is ideally suited for the study of light controlled processes.
The PhD students in this project area will learn to develop and apply cutting edge femtosecond spectroscopy experiments. They will receive in depth training on femtosecond lasers, nonlinear optics and nonlinear spectroscopy as well as on advanced methods of data analysis and quantum chemistry. Through cooperation within CLiC they will become familiar with advanced methods of quantum dynamics and theoretical spectroscopy as well as with state of the art organic synthesis.
The group of Schwalbe will characterize protein and RNA folding utilizing caged compounds. In the first aim, new caged puromycin groups will be synthesized to study protein folding of nascent polypeptide chains when released from the ribosome. In a second aim, complex RNA folding reactions populating various intermediates will be studied. We will study the reactivity and conformational dynamics of a one-sequence-two-ribozymes construct developed in the Bartel group. In both projects, the application of light-triggered time-resolved NMR spectroscopy will be central.
RNA molecules traverse rugged energy landscapes when folding into functional structures. As such, they can easily become trapped in misfolded conformations. Proteins with RNA chaperone or RNA helicase activity modulate an RNA’s free-energy landscape in order to accelerate RNA folding. Within CLiC the Fürtig group wants to decipher the mechanism of RNA helicases and determine how they promote RNA folding reactions.
During the course of the thesis the PhD student will gain a deep knowledge of protein biochemistry and RNA biology. He/She will apply modern liquid state NMR technology to biomacromolecules and will be enabled to develop new NMR experiments when needed. The PhD student will learn to use a sophisticated coupled laser NMR system.
The group of Clemens Glaubitz will use uncaging strategies for triggering enzymatic events of proteins within a membrane environment. The enzymatic reactions will be characterized by time-resolved solid-state NMR. The group has a long-standing expertise in biological solid-state NMR and in particular in membrane protein biophysics. The Glaubitz group has studied membrane-bound light-receptors, transport proteins, GPCRs and kinases. Their solid-state NMR methodology relies on high-field techniques and is complemented by dynamic nuclear polarisation, which is used in a close interplay with biochemical approaches. Recently, the group has started to explore the use of time-resolved solid-state NMR for elucidating kinetic processes within and at the membrane.
The PhD student of this research area will be qualified in connected areas of expertise: He/She will be trained in solid-state NMR and in particular in fast detection techniques, in membrane protein biochemistry, in optical methods required to control light-triggered reactions and in preparing and handling of photo-labile compounds. The combination of these areas of expertise brings them into the unique position to develop novel approaches for observing kinetic events within biological membranes.
The Tampé lab has a long-term experience in the field of biochemistry and biophysics, as well as in the development in nano- and opto-chemical tools for the in-situ assembly of macromolecular complexes. The lab has engineered minimalistic photo-activatable lock-and-key elements with striking characteristics in terms of site-specific tracing, tracking and manipulation of proteins and cellular pathways by light.
PhD students of this project area will be synergistically integrated into true transdisciplinary approaches reaching from organic synthesis, chemical biology, protein biochemistry, molecular biology with outreach into molecular cell biology and medicine.
- "Three-dimensional protein networks assembled by two‐photon activation", V. Gatterdam, R. Ramadass, T. Stoess, M. A. H. Fichte, J. Wachtveitl, A. Heckel, R. Tampé, Angew. Chem. Int. Ed. 2014, 53, 5680-5684.
The Heilemann group develops super-resolution fluorescence microscopy techniques that are based on the single-molecule detection of photoswitchable fluorophores. The group applies super-resolution microscopy to visualize cellular structures at the nanoscale, to quantify stoichiometries in protein-clusters and to follow the dynamics of single biomolecules in live cells. In addition, the group applies a variety of other single-molecule techniques (e.g. single-molecule FRET) to study biomolecular structures and dynamics.
The PhD students will obtain extensive knowledge in new classes of photoactivatable fluorophores (through various caging mechanisms), their spectroscopic characterization at the single-molecule level and their application for super-resolution microscopy. They will further be trained in various other single-molecule spectroscopy and imaging techniques.
The expertise of the Morgner group is the development and application of mass spectrometry (MS) (www.LILBID.de). The non-covalent MS methods nano-electrospray (nESI) and Laser-Induced-Liquid-Bead-Ion-Desorption (LILBID) are especially suited to study biological samples like proteins, DNA and RNA. LILBID offers the possibility to study those samples time-resolved in physiological environment to gain information e.g. about the interactions of ligands to their binding partners or the oligomerization of proteins, especially membrane proteins.
The PhD student in this project will expand the LILBID technique to achieve time resolved (TR) measurements on fast sub-second time-scales. Therefore, the student will be trained in the use of mass spectrometers as well as handling and preparation of biological samples prior to MS analysis. He/she will learn to design the optimal geometry of instrumental parts by using the simulation software SIMION and the computer-aided design software AutoCAD which will be the base for manufacturing those components. Working closely with the PhD projects A2.3 and B1.2 he/she will run TR-LILBID-MS experiments and answer biological questions.